Learning objectives: To describe seeding ROIs to reproduce the language fascicles separately for preoperative mapping with DTI tractography.

Background: Tractography mapping for subcortical white matter fibers is nowadays worldwide available. However, a clear protocol for reconstruction of each fascicle separately has not been described yet.

 Protocol for Preoperative Subcortical Mapping with Fiber Tracking with Diffusion Tensor Imaging for Tumors in Eloquent Areas